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The human immunodeficiency virus (HIV) is the causative agent of acquired immune deficiency syndrome (AIDS). The general method of detecting infection with HIV is to observe the presence of antibodies to the virus by an EIA method followed by confirmation with Western Blot. The iCare Rapid Anti-HIV (1&2) Test is a simple, visual qualitative test that detects antibodies in human whole blood, serum or plasma. The test is based on immunochromatography and can give a result within 15 minutes.
When performing the HIV test, the HIV Test Kit instructions must be followed closely.
The assay starts with a diluent prepared sample applied to the sample well. The HIV antigen -colloidal gold conjugate embedded in the sample pad reacts with the HIV antibody present in blood, serum or plasma sample forming conjugate/HIV antibody complex. As the mixture is allowed to migrate along the test strip, the conjugate/HIV antibody complex is captured by a second antibody immobilized on a membrane forming a colored test band in the test region. A negative sample does not produce a test band due to the absence of colloidal gold conjugate/HIV antibody complex. The antigens used in the conjugate test are recombinant proteins that correspond to highly immunoreactive regions of HIV1 and HIV2. A colored control band in the control region appears at the end of test procedure regardless of test result. This control band is the result of colloidal gold conjugate binding to the anti-HIV antibody immobilized on the membrane. The control band indicates that the colloidal gold conjugate is functional.
If there has been a possible exposure to infected bodily fluids, and the person subsequently tested negative for HIV, the test must be repeated in 90 days. It is imperative that a positive HIV test result be followed by a Western Blot or PCR test that is performed by a doctor or clinic to confirm if you are indeed HIV positive. Since NO test, nor test kit is infallible, it is recommended to confirm test results by using a licensed medical testing facility. For one time usage only.
Don’t let that uneasy feeling bother you. Be affirmative and get yourself tested today.
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